TAYLOR E W, CRAMER W
Biophys J. 1963 Mar;3(2):143-54. doi: 10.1016/s0006-3495(63)86810-1.
The intrinsic birefringences of TMV, tropocollagen, and paramyosin were calculated from flow birefringence measurements using the theory of Peterlin and Stuart. The values are -0.029, -0.029, and -0.030, respectively. The intrinsic birefringences of TMV and tropocollagen were measured as a function of the refractive index of the solvent in glycerol-water mixtures. In both cases the values were not constant and became less negative as the refractive index increased. Theoretical calculations showed that the large solvent effect could not be caused by a hydration shell of index different from that of the bulk solvent. It is concluded that either (a) the intrinsic birefringence calculated from the Peterlin-Stuart theory is incorrect or (b) the intrinsic birefringence depends markedly on the solvent. These results are of importance to the problem of quantitative polarized light microscopy since the separation of form and intrinsic birefringence contributions is based on the assumption that intrinsic birefringence is independent of solvent.
利用彼得林和斯图尔特理论，通过流动双折射测量计算了烟草花叶病毒（TMV）、原胶原蛋白和副肌球蛋白的固有双折射。其值分别为-0.029、-0.029和-0.030。测量了TMV和原胶原蛋白的固有双折射随甘油-水混合物中溶剂折射率的变化情况。在这两种情况下，其值都不是恒定的，并且随着折射率的增加，负值变小。理论计算表明，大的溶剂效应不可能是由折射率不同于本体溶剂的水化层引起的。得出的结论是，要么（a）根据彼得林-斯图尔特理论计算出的固有双折射是不正确的，要么（b）固有双折射明显依赖于溶剂。这些结果对于定量偏振光显微镜问题很重要，因为形态和固有双折射贡献的分离是基于固有双折射与溶剂无关这一假设的。
