Fitzsimmons T D, Fagerholm P, Härfstrand A, Schenholm M
Department of Ophthalmology, Karolinska Institutet, S:t Eriks Eye Hospital, Stockholm, Sweden.
Invest Ophthalmol Vis Sci. 1992 Oct;33(11):3011-6.
Hyaluronic acid (HA) is not normally found in the corneal stroma. Rabbit corneas were examined for the presence of stromal HA after excimer laser treatment. One eye in each of 28 rabbits received a 60 microns deep superficial keratectomy with the excimer laser. After 1, 8, 21, and 60 days, the corneas were analyzed by quantitative and histochemical methods specific for HA. A statistically significant increase in the HA concentration compared to the baseline amount in the untreated fellow eye was seen at 8, 21, and 60 days. HA was visualized histochemically in the anterior stroma of the excimer-treated eyes at all times tested. The presence of HA after excimer surgery may influence the hydration, thickness, and transparency of the cornea. The reactive production of HA in the stroma may represent a nonspecific corneal tissue response to injury.
透明质酸（HA）通常不存在于角膜基质中。研究人员对接受准分子激光治疗后的兔角膜进行检查，以确定基质中是否存在HA。28只兔子中，每只兔子的一只眼睛接受了60微米深度的准分子激光浅层角膜切除术。在术后1天、8天、21天和60天，通过针对HA的定量和组织化学方法对角膜进行分析。在第8天、21天和60天，与未治疗的对侧眼睛的基线量相比，HA浓度有统计学意义的增加。在所有测试时间点，经准分子激光治疗的眼睛的前基质中均可通过组织化学方法观察到HA。准分子激光手术后HA的存在可能会影响角膜的水合作用、厚度和透明度。基质中HA的反应性产生可能代表角膜组织对损伤的非特异性反应。
