Drubaix I, Legeais J M, Robert L, Renard G
INSERM U86, Département d'Ophtalmologie, Hôtel-Díeu de Paris, France.
Exp Eye Res. 1997 Mar;64(3):301-4. doi: 10.1006/exer.1996.0212.
The hyaluronan content during wound healing following excimer laser photoablation was investigated. Rabbit corneas were photoablated (Summit Omnimed, 193 nm, 5 mm diameter, 50 microns and 100 microns depth). Central optical zones of photoablated and contralateral corneas were removed 7, 30 and 90 days after surgery. Corneas from 2 untreated rabbits were used as control. Hyaluronan content was determined after pepsin-solubilization using an alkaline-phosphatase linked hyaluronectin sorbent assay. The hyaluronan content of non-photoablated contralateral corneas was significantly increased and the changes appeared depth dependent. Hyaluronan content returned to control levels by the first month at 50 microns depth and by the third month at 100 microns depth in contralateral corneas. The hyaluronan content of the photoablated corneas was not significantly different from that of the non-photoablated contralateral sample regardless of the depth of photoablation. By contrast, the hyaluronan content of the treated corneas was significantly higher than that of the controls during the first month post-surgery and returned to control values by the third month. Thus the hyaluronan contents in the photoablated and contralateral corneas after excimer laser corneal photoablation were very similar during wound healing. This study shows that excimer laser photoablation induced transient depth-dependent contralateral alterations of the hyaluronan content.
研究了准分子激光光蚀术后伤口愈合过程中的透明质酸含量。对兔角膜进行光蚀（Summit Omnimed，193nm，直径5mm，深度50微米和100微米）。术后7天、30天和90天切除光蚀角膜和对侧角膜的中央光学区。使用2只未经处理的兔的角膜作为对照。采用碱性磷酸酶连接的透明质酸结合蛋白吸附测定法，在胃蛋白酶溶解后测定透明质酸含量。未光蚀的对侧角膜的透明质酸含量显著增加，且变化呈深度依赖性。对侧角膜在50微米深度时，第一个月透明质酸含量恢复到对照水平；在100微米深度时，第三个月恢复到对照水平。无论光蚀深度如何，光蚀角膜的透明质酸含量与未光蚀的对侧样本相比无显著差异。相比之下，治疗后角膜的透明质酸含量在术后第一个月显著高于对照组，第三个月恢复到对照值。因此，准分子激光角膜光蚀术后光蚀角膜和对侧角膜在伤口愈合过程中的透明质酸含量非常相似。本研究表明，准分子激光光蚀诱导了透明质酸含量的短暂的、深度依赖性的对侧改变。
