Kolchinskaia L I, Lishko V K, Malysheva M K
Biokhimiia. 1976 May;41(5):933-8.
Effects of ATP, acetyl phosphate (AcP) and p-nitrophenyl phosphate (p-NPP) on the inhibition of the Na+, K+-ATPase activity were studied. ATP, AcP and p-NPP were found to facilitate the ouabain-induced inhibition of the enzyme activity only after the injection of these phosphorylyzing agents into the erythrocyte ghosts. Inside the ghosts Na+ ions enhanced the effects of the phosphorylyzing agents. K+ ions in the environment removed the stimulating effects of ATP, AcP and p-NPP on the ouabain-induced inhibition of Na+, K+-ATPase activity. It is concluded that the sites of AcP and p-NPP hydrolysis as well as the active center for ATP are localized on the inner surface of the cell membrane.
研究了三磷酸腺苷（ATP）、乙酰磷酸（AcP）和对硝基苯磷酸酯（p-NPP）对钠钾-ATP酶活性抑制的影响。仅在将这些磷酸化剂注射到红细胞血影后，才发现ATP、AcP和p-NPP促进哇巴因诱导的酶活性抑制。在血影内部，钠离子增强了磷酸化剂的作用。环境中的钾离子消除了ATP、AcP和p-NPP对哇巴因诱导的钠钾-ATP酶活性抑制的刺激作用。得出结论，AcP和p-NPP的水解位点以及ATP的活性中心位于细胞膜的内表面。
