用标记的抗人血型A单克隆抗体对小鼠自发性乳腺癌进行特异性生物检测。

Specific biodetection of a murine spontaneous mammary carcinoma with labelled anti-human blood group-A monoclonal antibody.

作者信息

Horenstein A L, Koss A, Colombo L L, Glait H M

机构信息

Oncologic Center of Nuclear Medicine, National Atomic Energy Commission, Buenos Aires, Argentina.

出版信息

Int J Rad Appl Instrum B. 1992 Jul;19(5):589-96. doi: 10.1016/0883-2897(92)90154-q.

DOI:10.1016/0883-2897(92)90154-q

PMID:1399688

Abstract

The expression of cell surface antigens of the spontaneous transplantable M3-murine tumour was studied by means of the monoclonal antibody (MAb) B2C114 which recognizes the human blood group-A carbohydrate antigen. Following radioiodination the MAb retained their immunoreactivity and demonstrated a significantly higher in vitro binding with isolated M3-tumour cells as compared with a control antibody. B2C114 revealed 10(6) antigenic sites per cell, with a constant affinity of 5.1 x 10(9)/M. Biodistribution studies showed that B2C114 discriminated between malignant tumour and mouse normal tissues. Radioimmunodetection of Balb/c mice bearing s.c. M3-tumour showed that tumour was specifically defined without subtraction 1 day after injection of the radiolabelled antibody.

摘要

利用识别人类血型A碳水化合物抗原的单克隆抗体（MAb）B2C114，对自发可移植性M3 - 小鼠肿瘤的细胞表面抗原表达进行了研究。放射性碘化后，单克隆抗体保留了其免疫反应性，并且与对照抗体相比，显示出与分离的M3 - 肿瘤细胞在体外的结合显著更高。B2C114显示每个细胞有10⁶个抗原位点，恒定亲和力为5.1×10⁹/M。生物分布研究表明，B2C114能够区分恶性肿瘤和小鼠正常组织。对皮下接种M3 - 肿瘤的Balb/c小鼠进行放射免疫检测显示，注射放射性标记抗体1天后，无需扣除即可特异性地确定肿瘤。