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The mechanism of the delta5-3-ketosteroid isomerase reaction: absorption and fluorescence spectra of enzyme-steroid complexes.

作者信息

WANG S F, KAWAHARA F S, TALALAY P

出版信息

J Biol Chem. 1963 Feb;238:576-85.

PMID:13998799
Abstract
摘要

相似文献

1
The mechanism of the delta5-3-ketosteroid isomerase reaction: absorption and fluorescence spectra of enzyme-steroid complexes.δ5-3-酮类固醇异构酶反应的机制：酶-类固醇复合物的吸收光谱和荧光光谱
J Biol Chem. 1963 Feb;238:576-85.
2
Human placental 3beta-hydroxysteroid dehydrogenase: delta5-isomerase. Demonstration of an intermediate in the conversion of 3beta-hydroxypregn-5-en-20-one to pregn-4-ene-3,20-dione.人胎盘3β-羟基类固醇脱氢酶：δ5-异构酶。3β-羟基孕-5-烯-20-酮转化为孕-4-烯-3,20-二酮过程中一种中间体的证明。
J Biol Chem. 1976 Mar 25;251(6):1632-8.
3
Characterization of monoclinic crystal form of an enzyme of steroid metabolism, delta5-3-ketosteroid isomerase.
J Mol Biol. 1976 May 25;103(3):665-7. doi: 10.1016/0022-2836(76)90225-4.
4
Binding of steroids to P. testosteroni delta5-3-ketosteroid isomerase: measurement of the number of binding sites by equilibrium dialysis.
Biochem Biophys Res Commun. 1978 Dec 14;85(3):1082-9. doi: 10.1016/0006-291x(78)90653-8.
5
Enzymatic and nonenzymatic polarizations of alpha,beta-unsaturated ketosteroids and phenolic steroids. Implications for the roles of hydrogen bonding in the catalytic mechanism of delta 5-3-ketosteroid isomerase.α,β-不饱和酮甾体和酚类甾体的酶促极化与非酶促极化。对氢键在δ5-3-酮甾体异构酶催化机制中作用的启示。
Biochemistry. 1995 Jan 17;34(2):426-34. doi: 10.1021/bi00002a006.
6
NMR studies of the secondary structure in solution and the steroid binding site of delta5-3-ketosteroid isomerase in complexes with diamagnetic and paramagnetic steroids.溶液中二级结构以及δ5-3-酮甾体异构酶与抗磁性和顺磁性甾体形成的复合物中甾体结合位点的核磁共振研究。
Biochemistry. 1997 Mar 25;36(12):3458-72. doi: 10.1021/bi962844u.
7
Irreversible active-site-directed inhibition of delta 5-3-ketosteroid isomerase by steroidal 17-beta-oxiranes. Evidence for two modes of binding in steroid-enzyme complexes.
Biochem Biophys Res Commun. 1980 Aug 14;95(3):1131-7. doi: 10.1016/0006-291x(80)91590-9.
8
Characterization of hexagonal crystal form of an enzyme of steroid metabolism, delta5-3-ketosteroid isomerase: a new method of crystal density measurement.
J Mol Biol. 1976 May 25;103(3):659-64. doi: 10.1016/0022-2836(76)90224-2.
9
Irreversible inactivation of delta 5-3-ketosteroid isomerase of Pseudomonas testosteroni by acetylenic suicide substrates. Mechanism of formation and properties of the steroid-enzyme adduct.乙炔基自杀底物对睾丸酮假单胞菌δ5-3-酮类固醇异构酶的不可逆失活作用。类固醇-酶加合物的形成机制及性质
J Biol Chem. 1981 Jul 10;256(13):6842-50.
10
Interaction of steroids with Pseudomonas testosteroni 3-oxosteroid delta4--delta5-isomerase.
Biochemistry. 1977 Nov 15;16(23):5045-53. doi: 10.1021/bi00642a016.

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Oriented internal electrostatic fields: an emerging design element in coordination chemistry and catalysis.定向内部静电场：配位化学和催化领域中一种新兴的设计元素。
Chem Sci. 2022 Apr 20;13(19):5432-5446. doi: 10.1039/d2sc01715f. eCollection 2022 May 18.
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Electric Fields and Enzyme Catalysis.电场与酶催化
Annu Rev Biochem. 2017 Jun 20;86:387-415. doi: 10.1146/annurev-biochem-061516-044432. Epub 2017 Mar 24.
3
The isomerization of Δ5-androstene-3,17-dione by the human glutathione transferase A3-3 proceeds via a conjugated heteroannular diene intermediate.
人谷胱甘肽转移酶A3-3催化的Δ5-雄甾烯-3,17-二酮异构化反应通过一个共轭杂环二烯中间体进行。
J Biol Chem. 2014 Nov 14;289(46):32243-32252. doi: 10.1074/jbc.M114.601609. Epub 2014 Sep 23.
4
Pathogen roid rage: cholesterol utilization by Mycobacterium tuberculosis.病原体的类固醇狂热：结核分枝杆菌对胆固醇的利用
Crit Rev Biochem Mol Biol. 2014 Jul-Aug;49(4):269-93. doi: 10.3109/10409238.2014.895700. Epub 2014 Mar 10.
5
FLUORESCENCE OF SOLUTIONS: A REVIEW.溶液荧光：综述
J Clin Pathol. 1964 Jul;17(4):371-94. doi: 10.1136/jcp.17.4.371.
6
Roles of dimerization in folding and stability of ketosteroid isomerase from Pseudomonas putida biotype B.二聚化在恶臭假单胞菌生物型B的酮甾体异构酶折叠和稳定性中的作用。
Protein Sci. 2001 Apr;10(4):741-52. doi: 10.1110/ps.18501.
7
NMR evidence for the participation of a low-barrier hydrogen bond in the mechanism of delta 5-3-ketosteroid isomerase.核磁共振证据表明低势垒氢键参与δ5-3-酮甾体异构酶的作用机制。
Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8220-4. doi: 10.1073/pnas.93.16.8220.
8
Inactivation of delta 5-3-oxo steroid isomerase with active-site-directed acetylenic steroids.用活性位点导向的炔类甾体使δ5-3-氧代甾体异构酶失活。
Biochem J. 1981 Jan 1;193(1):217-27. doi: 10.1042/bj1930217.
9
Mutants defective in isomerase and decarboxylase activities of the 4-hydroxyphenylacetic acid meta-cleavage pathway in Pseudomonas putida.恶臭假单胞菌中4-羟基苯乙酸间位裂解途径的异构酶和脱羧酶活性存在缺陷的突变体。
J Bacteriol. 1980 May;142(2):480-5. doi: 10.1128/jb.142.2.480-485.1980.
10
The behavior of sterols on silica surfaces and at other interfaces.
J Am Oil Chem Soc. 1966 May;43(5):275-80. doi: 10.1007/BF02609673.