WATSON J D
Science. 1963 Apr 5;140(3562):17-26. doi: 10.1126/science.140.3562.17.
We can now have considerable confidence that the broad features of protein synthesis are understood. The involvement of RNA is very much more complicated than was imagined in 1953. There is not one functional RNA. Instead, protein synthesis demands the ordered interaction of three classes of RNA-ribosomal, soluble, and messenger. Many important questions, however, remain unanswered. For instance, there is no theoretical framework for the ribosomal subunits, nor for that matter, do we understand the functional significance of ribosomal RNA. Most satisfying is the realization that all the steps in protein replication will be shown to involve well-understood chemical forces. As yet we do not know all the details. For example, are the DNA base pairs involved in messenger RNA selection of the corresponding amino-acyl-sRNA? With luck, this will soon be known. We can thus have every expectation that future progress in understanding selective protein synthesis (and its consequences for embryology) will have a similarly well-defined and, when understood, easy-to-comprehend chemical basis (62).
我们现在可以相当有信心地认为，蛋白质合成的大致过程已为人所知。RNA的参与比1953年人们想象的要复杂得多。并非只有一种功能性RNA。相反，蛋白质合成需要三类RNA（核糖体RNA、可溶性RNA和信使RNA）有序相互作用。然而，许多重要问题仍未得到解答。例如，对于核糖体亚基没有理论框架，就此而言，我们也不理解核糖体RNA的功能意义。最令人满意的是认识到蛋白质复制的所有步骤都将被证明涉及已充分理解的化学作用力。目前我们还不知道所有细节。例如，信使RNA选择相应的氨酰基可溶性RNA时，DNA碱基对是否参与其中？幸运的话，很快就会知晓。因此，我们完全有理由期待，未来在理解选择性蛋白质合成（及其对胚胎学的影响）方面取得的进展将有同样明确且易懂的化学基础（62）。
