Sallustio B C, Morris R G, Horowitz J D
Department of Clinical Pharmacology, Queen Elizabeth Hospital, Woodville South, South Australia.
J Chromatogr. 1992 May 8;576(2):321-7. doi: 10.1016/0378-4347(92)80206-6.
Two high-performance liquid chromatographic analytical methods have been developed for the measurement of dl-sotalol or d-sotalol and l-sotalol in plasma, using dl-atenolol as internal standard. Quantitation of dl-sotalol was carried out, following solid-phase extraction, on a 5-microns C18 reversed-phase column, with a mobile phase containing acetonitrile, ion-pairing reagent and distilled water, using ultraviolet detection at 235 nm. Quantitation of d-sotalol and l-sotalol was based on derivatisation with the chiral agent S-(-)-alpha-methylbenzyl isocyanate, followed by chromatographic separation on a 3-microns C18 reversed-phase column, with a mobile phase containing methanol, glacial acetic acid and distilled water, with fluorimetric detection at 220 nm excitation and 300 nm emission. A preliminary application of the latter method suggests that the disposition of sotalol in humans is not enantioselective.
已开发出两种高效液相色谱分析方法，用于测定血浆中的消旋索他洛尔或右旋索他洛尔和左旋索他洛尔，以内消旋阿替洛尔作为内标。在进行固相萃取后，于5微米的C18反相柱上对消旋索他洛尔进行定量，流动相包含乙腈、离子对试剂和蒸馏水，采用235nm的紫外检测。右旋索他洛尔和左旋索他洛尔的定量基于用手性试剂S-(-)-α-甲基苄基异氰酸酯进行衍生化，随后在3微米的C18反相柱上进行色谱分离，流动相包含甲醇、冰醋酸和蒸馏水，采用在220nm激发和300nm发射波长下的荧光检测。后一种方法的初步应用表明，索他洛尔在人体内的处置不存在对映体选择性。
