Shimizu T, Hiraoka M, Nakanomyo H
Kanagawa Laboratories, Bristol-Myers Squibb, Japan.
J Chromatogr B Biomed Appl. 1995 Dec 1;674(1):77-83. doi: 10.1016/0378-4347(95)00304-5.
A simple and sensitive high-performance liquid chromatographic (HPLC) method for the determination of (+)-(S)-sotalol and (-)-(R)-sotalol in biological fluids was established. Following extraction with isopropyl alcohol from biological samples on a Sep-Pak C18 cartridge, the eluent was derivatized with 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate (GITC). The diastereoisomeric derivates were resolved by HPLC with UV detection at 225 nm. Calibration was linear from 0.022 to 4.41 micrograms/ml in human plasma and from 0.22 to 88.2 micrograms/ml in human urine for both (+)-(S)- and (-)-(R)-sotalol. The lower limit of determination was 0.022 microgram/ml for plasma and 0.22 microgram/ml for urine. The within-day and day-to-day coefficients of variation were less than 7.5% for each enantiomer at 0.09 and 1.8 microgram/ml in plasma and at 0.44 and 4.4 micrograms/ml in urine. The method is also applicable to other biological specimens such as rat, mouse and rabbit plasma.
建立了一种简单、灵敏的高效液相色谱（HPLC）法，用于测定生物样品中（+）-（S）-索他洛尔和（-）-（R）-索他洛尔。生物样品在Sep-Pak C18柱上用异丙醇萃取后，洗脱液用2,3,4,6-四-O-乙酰基-β-D-吡喃葡萄糖基异硫氰酸酯（GITC）衍生化。通过HPLC在225nm处进行紫外检测分离非对映异构体衍生物。（+）-（S）-和（-）-（R）-索他洛尔在人血浆中的校准曲线在0.022至4.41微克/毫升范围内呈线性，在人尿中的校准曲线在0.22至88.2微克/毫升范围内呈线性。血浆的测定下限为0.022微克/毫升，尿液的测定下限为0.22微克/毫升。在血浆中0.09和1.8微克/毫升以及尿液中0.44和4.4微克/毫升时，每种对映体的日内和日间变异系数均小于7.5%。该方法也适用于其他生物样品，如大鼠、小鼠和兔的血浆。
