Fiset C, Philippon F, Gilbert M, Turgeon J
Ecole de Pharmacie, Université Laval, Ste-Foy, Quebec, Canada.
J Chromatogr. 1993 Feb 26;612(2):231-7. doi: 10.1016/0378-4347(93)80168-4.
A high-performance liquid chromatographic (HPLC) assay for determination of sotalol enantiomers in biological fluids was developed to assess the stereoselective disposition of the drug in man. Following extraction at pH 9.0 with a mixture of chloroform-isopropanol (3:1, v/v), the organic phase was evaporated to dryness and the residue derivatized with (-)-methyl chloroformate. Diastereoisomeric derivatives were resolved by HPLC (C8 column) with fluorescence detection (lambda ex = 235 nm and lambda em = 300 nm). Retention times of l- and d-sotalol derivatives were 13 and 15 min while that of the internal standard, S-(-)-atenolol, was 12.3 min. The detection limit of each enantiomer was 12.5 ng/ml using 1 ml of plasma or urine. Intra-day and inter-day coefficients of variation were less than 10% for each enantiomer in the range 0.125-2.5 micrograms/ml in plasma and 0.25-2.5 micrograms/ml in urine.
建立了一种用于测定生物流体中索他洛尔对映体的高效液相色谱（HPLC）分析方法，以评估该药物在人体内的立体选择性处置。在pH 9.0条件下，用氯仿 - 异丙醇混合物（3:1，v/v）萃取后，将有机相蒸发至干，残余物用（-）-氯甲酸甲酯衍生化。非对映异构体衍生物通过HPLC（C8柱）结合荧光检测（激发波长λex = 235 nm，发射波长λem = 300 nm）进行分离。左旋和右旋索他洛尔衍生物的保留时间分别为13分钟和15分钟，而内标S - （-） - 阿替洛尔的保留时间为12.3分钟。使用1 ml血浆或尿液时，每种对映体的检测限为12.5 ng/ml。在血浆中0.125 - 2.5μg/ml以及尿液中0.25 - 2.5μg/ml的范围内，每种对映体的日内和日间变异系数均小于10%。
