Landes B D, Miscoria G, Flouvat B
Laboratoire de Toxicologie et de Pharmacocinétique, Hôpital Ambroise Paré, Boulogne Billancourt, France.
J Chromatogr. 1992 May 20;577(1):117-22. doi: 10.1016/0378-4347(92)80605-p.
A high-performance liquid chromatographic method for the simultaneous determination of lansoprazole, a new proton pump inhibitor, and its metabolites in human plasma is described. Lansoprazole, its metabolites and an internal standard were extracted with tert.-butyl methyl ether. Samples were injected using an automatic injector via a loop column, and separation was obtained using a reversed-phase column under isocratic conditions. The absorbance was monitored at 285 and 303 nm. The quantification limit was 2 ng/ml for lansoprazole and 3 or 5 ng/ml for the metabolites. No endogenous compounds were found to interfere. The mean overall recovery was between 75 and 95% for lansoprazole and its metabolites. This method is suitable for pharmacokinetic studies.
本文描述了一种高效液相色谱法，用于同时测定人体血浆中新型质子泵抑制剂兰索拉唑及其代谢物。兰索拉唑、其代谢物和内标用叔丁基甲醚萃取。样品通过定量环柱由自动进样器进样，在等度条件下使用反相柱进行分离。在285和303nm处监测吸光度。兰索拉唑的定量限为2ng/ml，代谢物的定量限为3或5ng/ml。未发现内源性化合物干扰。兰索拉唑及其代谢物的平均总回收率在75%至95%之间。该方法适用于药代动力学研究。
