Avgerinos A, Karidas T h, Potsides C, Axarlis S
Military Pharmaceutical Laboratories, Athens, Greece.
Eur J Drug Metab Pharmacokinet. 1998 Apr-Jun;23(2):329-32. doi: 10.1007/BF03189359.
A simple and rapid (extractionless) high-performance liquid chromatographic method with UV detection at 230 nm was developed for the determination of lansoprazole in biological fluids and pharmaceutical dosage. Niflumic acid was added as internal standard. The separation was performed at ambient temperature on a C18 Spherisorb column with acetonitrile + 0.1 M sodium acetate (40:60, v/v, pH 7) as mobile phase. The retention time was 5.2 min for niflumic acid and 6.7 min for lansoprazole. The detection limit was 20 ng/ml using a 100 microl loop. The method was successfully applied to a pharmacokinetic study of lansoprazole in humans.
建立了一种简单快速（无需萃取）的高效液相色谱法，用于测定生物体液和药物制剂中的兰索拉唑，采用230 nm紫外检测。加入尼氟酸作为内标。在室温下，使用C18 Spherisorb柱，以乙腈+0.1 M醋酸钠（40:60，v/v，pH 7）作为流动相进行分离。尼氟酸的保留时间为5.2分钟，兰索拉唑的保留时间为6.7分钟。使用100微升定量环时，检测限为20 ng/ml。该方法成功应用于兰索拉唑在人体的药代动力学研究。
