Karol M D, Granneman G R, Alexander K
Drug Metabolism Department, Abbott Laboratories, Abbott Park, IL 60064-3500, USA.
J Chromatogr B Biomed Appl. 1995 Jun 9;668(1):182-6. doi: 10.1016/0378-4347(95)00068-t.
A high-performance liquid chromatographic method for the determination of lansoprazole, a new proton-pump inhibitor, and five of its metabolites in human plasma is described. Lansoprazole, its metabolites, and internal standard (omeprazole) were extracted into diethyl ether-methylene chloride and separation was obtained using a reversed-phase column under isocratic conditions. The method features monochromatic ultraviolet detection at 285 nm, and single extraction, single evaporation sample handling. The lower limit of quantitation, based on standards with acceptable coefficients of variation, was 10 ng/ml for all compounds. No endogenous compounds were found to interfere. This method has been demonstrated to be suitable for pharmacokinetic studies in humans.
本文描述了一种用于测定新型质子泵抑制剂兰索拉唑及其五种代谢物在人血浆中含量的高效液相色谱法。兰索拉唑及其代谢物以及内标（奥美拉唑）被萃取到乙醚 - 二氯甲烷中，并在等度条件下使用反相柱进行分离。该方法的特点是在285nm处进行单色紫外检测，以及单萃取、单蒸发的样品处理方式。基于变异系数可接受的标准品，所有化合物的定量下限均为10ng/ml。未发现内源性化合物干扰。该方法已被证明适用于人体药代动力学研究。
