Mardens Y, Kumps A, Planchon C, Wurth C
Laboratory of Medical Biochemistry, Free University of Brussels (ULB), Belgium.
J Chromatogr. 1992 Jun 10;577(2):341-6. doi: 10.1016/0378-4347(92)80256-p.
We have compared a new isolation procedure for urinary organic acids using strong anion-exchange columns with a solvent partition (ethyl acetate) method. Urinary samples from two healthy children and from nine children with organic acidurias were analysed by both procedures. Although the solid-phase extraction is more efficient for polyhydroxy acids, some polar acids, and some glycine derivatives, clinically important compounds such as oxalic, methylcitric, pyruvic, glyoxylic and 2-ketoglutaric acids, are not recovered or are only poorly recovered. However, both procedures may be used as a routine method for the diagnosis of the organic acidurias included in this study.
我们将一种使用强阴离子交换柱分离尿中有机酸的新方法与溶剂分配（乙酸乙酯）法进行了比较。用这两种方法分析了来自两名健康儿童和九名患有有机酸尿症儿童的尿样。虽然固相萃取对多羟基酸、一些极性酸和一些甘氨酸衍生物的提取效率更高，但一些临床上重要的化合物，如草酸、甲基柠檬酸、丙酮酸、乙醛酸和2-酮戊二酸，无法回收或回收效果很差。然而，这两种方法都可用作本研究中所包含的有机酸尿症诊断的常规方法。
