Tanaka K, West-Dull A, Hine D G, Lynn T B, Lowe T
Clin Chem. 1980 Dec;26(13):1847-53.
A gas-chromatographic method for urinary organic acid analysis is described, designed to be used routinely for the diagnosis of organic aciduria. It involves extraction of urine with ethyl acetate, dehydration of extract residues, and trimethylsilylation. Organic acids are identified by using an extensive list of retention indices published in the accompanying paper (this issue). Quantitative values are given for organic acids in urines from 50 ostensibly normal subjects. Typical chromatograms of urinary organic acids from patients with eight well-established organic acidurias are also shown.
本文描述了一种用于尿有机酸分析的气相色谱法，该方法旨在常规用于有机酸尿症的诊断。它包括用乙酸乙酯提取尿液、提取物残渣脱水以及三甲基硅烷化。通过使用随附论文（本期）中公布的大量保留指数列表来鉴定有机酸。给出了50名表面上正常受试者尿液中有机酸的定量值。还展示了患有8种已明确的有机酸尿症患者的尿有机酸典型色谱图。
