Niwa T, Maruyama W, Nakahara D, Takeda N, Yoshizumi H, Tatematsu A, Takahashi A, Dostert P, Naoi M, Nagatsu T
Department of Internal Medicine, Nagoya University Branch Hospital, Japan.
J Chromatogr. 1992 Jul 1;578(1):109-15. doi: 10.1016/0378-4347(92)80231-e.
N-Methylsalsolinol, an analogue of 1,2,3,6-tetrahydropyridine, is present in the brains of patients with Parkinson's disease. To determine the metabolic pathway for the synthesis of N-Methylsalsolinol in the brain, salsolinol was perfused through the striatum or the substantia nigra of the rat brain by in vivo microdialysis. N-Methylsalsolinol was detected in the brain dialysate samples during microdialysis with salsolinol using gas chromatography-mass spectrometry with selected-ion monitoring. These results demonstrate that endogenous N-methylation of salsolinol into N-methylsalsolinol occurs in the brain in vivo.
N-甲基去甲猪毛菜酚，一种1,2,3,6-四氢吡啶的类似物，存在于帕金森病患者的大脑中。为了确定大脑中N-甲基去甲猪毛菜酚的合成代谢途径，通过体内微透析将去甲猪毛菜酚灌注到大鼠脑的纹状体或黑质中。在使用选择离子监测的气相色谱-质谱联用仪对去甲猪毛菜酚进行微透析期间，在脑透析液样本中检测到了N-甲基去甲猪毛菜酚。这些结果表明，去甲猪毛菜酚在体内大脑中发生内源性甲基化生成N-甲基去甲猪毛菜酚。
