免疫黏附研究。I. 用大米淀粉系统滴定人补体。
Studies with immune-adherence. I. Titration of human complement with a rice starch system.
作者信息
WOODWORTH H C
出版信息
Immunology. 1962 Jan;5(1):181-6.
Abstract
A method has been developed and standardized for the titration of human complement using the immune-adherence system with rice starch as the antigen. When starch granules are presensitized with normally occurring antibody and react with serum dilutions, the endpoint as determined by 50 per cent adherence yields an estimate of complement titre, reproducible within 20 per cent. This system produces titres of about 400 C′I-A units per ml. with human serum, and about 200 C′I-A units per ml. with guinea-pig serum.
摘要
已经开发并标准化了一种使用免疫粘附系统以大米淀粉作为抗原滴定人补体的方法。当淀粉颗粒用正常存在的抗体预先致敏并与血清稀释液反应时，由50%粘附确定的终点可得出补体滴度的估计值，其重现性在20%以内。该系统用人血清产生的滴度约为每毫升400 C′I-A单位，用豚鼠血清产生的滴度约为每毫升200 C′I-A单位。
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