Rosenberg M, Berger S A, Barki M, Goldberg S, Fink A, Miskin A
Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
J Clin Microbiol. 1992 Oct;30(10):2686-91. doi: 10.1128/jcm.30.10.2686-2691.1992.
The Diaslide urine culture device consists of a hinged case containing two opposing agar media separated by a sampler with a handle at one end and two bent sampler tips at the opposite end. The tips of the sampler are first dipped into the urine. The sampler is then pulled out through the casing, simultaneously inoculating both agar surfaces with a streaking dilution. As a result, individual colonies can be observed even when bacterial concentrations exceed 10(6) CFU/ml. The number of colonies on the Diaslide correlated linearly with CFU per milliliter as determined by dilution plating. The clinical performance of the Diaslide was compared with those of ordinary dipslides and conventional cultures with a sample of 473 prescreened hospital urine specimens. The sensitivity, specificity, and positive predictive value of Diaslide versus those of culture at the 10(4)-CFU/ml cutoff level were 97.5, 98.3, and 98.3%, respectively, compared with 98.8, 95.7, and 97.2%, respectively, for dipslide versus culture. Similar results were found at the 10(5)-CFU/ml cutoff level. Only 5.5% of the Diaslides required subculturing, compared with 14.7 and 9.4% of the dipslides and conventional cultures, respectively. The Diaslide proved more convenient than an ordinary dipslide for sampling low volumes of urine. These data suggest that the Diaslide is a simple, effective device for culturing of urine specimens.
Diaslide尿液培养装置由一个铰接的盒子组成，盒内有两个相对的琼脂培养基，中间由一个采样器隔开，采样器一端有一个手柄，另一端有两个弯曲的采样头。首先将采样头浸入尿液中。然后将采样器从盒中抽出，同时在两个琼脂表面进行划线稀释接种。因此，即使细菌浓度超过10⁶CFU/ml，也能观察到单个菌落。Diaslide上的菌落数与通过稀释平板法测定的每毫升CFU呈线性相关。用473份预先筛选的医院尿液标本样本，将Diaslide的临床性能与普通浸片和传统培养方法进行了比较。在10⁴CFU/ml的临界值水平下，Diaslide相对于培养法的敏感性、特异性和阳性预测值分别为97.5%、98.3%和98.3%，而浸片相对于培养法的相应数值分别为98.8%、95.7%和97.2%。在10⁵CFU/ml的临界值水平下也发现了类似结果。只有5.5%的Diaslide需要进行传代培养，而浸片和传统培养法的这一比例分别为14.7%和9.4%。事实证明，Diaslide在采集少量尿液样本时比普通浸片更方便。这些数据表明，Diaslide是一种用于尿液标本培养的简单、有效的装置。
