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浸式玻片尿液培养物的室温孵育

Room temperature incubation of dipslide urine cultures.

作者信息

Rosenthal S L, Freundlich L F

出版信息

Urology. 1976 Sep;8(3):251-253. doi: 10.1016/0090-4295(76)90378-2.

Abstract

Two hundred and fifty urine specimens selected at random were cultured simultaneously by routine methods and on dipslides containing MacConkey agar and cystine-lactose electrolyte deficient agar with colistin (CLED-colistin agar) at room temperature incubation. There were six major and five minor differences between the two methods in interpretation of results. Many of these differences were due to the fact that CLED-colistin agar fails to support the growth of many gram-positive species, and that the dipslide tended to overestimate the colony count. Nevertheless there was sufficient agreement between the two methods to suggest that dipslides can be used effectively in office or clinic without the need for an incubator.

摘要

随机选取250份尿液标本,分别采用常规方法以及在含有麦康凯琼脂和含多粘菌素的胱氨酸-乳糖电解质缺乏琼脂(CLED-多粘菌素琼脂)的浸式载玻片上,于室温孵育条件下同时进行培养。两种方法在结果判读上存在6处主要差异和5处次要差异。这些差异中有许多是由于CLED-多粘菌素琼脂无法支持许多革兰氏阳性菌的生长,以及浸式载玻片往往会高估菌落计数。然而,两种方法之间有足够的一致性,表明浸式载玻片可在办公室或诊所有效使用,无需培养箱。

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