对Cult-Dip Plus浸涂玻片法用于尿路感染的评估。
Evaluation of the Cult-Dip Plus dip slide method for urinary tract infection.
作者信息
Blondeau J M, Yaschuk Y, Galenzoski D, Hrabok D, Isaacson M, Lee L, Link H, Walshaw L
机构信息
Division of Microbiology, St Paul's Hospital, Saskatoon, Saskatchewan, Canada.
出版信息
J Clin Pathol. 1995 Aug;48(8):710-3. doi: 10.1136/jcp.48.8.710.
AIM
To evaluate the Cult-Dip Plus (Merck, Germany), a bacteriological culture test for detecting uropathogens.
METHODS
Cult-Dip Plus consists of Brolacin (CLED) and MacConkey agar, each containing methylumbelliferylglucuronide (MUG). Using 1022 urine samples, this product was compared with the routine method of calibrated loop inoculated CLED and blood agar for screening urine for uropathogens. The MUG test for identifying Escherichia coli was also evaluated.
RESULTS
Compared with the routine method, Cult-Dip Plus has a sensitivity, specificity, positive predictive value and negative predictive value of 88.3%, 98.0%, 91.9%, and 97.1%, respectively. The MUG test correctly identified 92% of E coli isolates with a sensitivity, specificity and positive predictive value of 91.6%, 95.2%, and 93.6%, respectively.
CONCLUSION
Cult-Dip Plus appears to be an alternative method to the calibrate loop method for detecting uropathogens. The MUG test permits rapid, reliable and inexpensive identification of E coli.
目的
评估用于检测尿路病原体的细菌培养测试Cult-Dip Plus（德国默克公司）。
方法
Cult-Dip Plus由含有甲基伞形酮葡糖苷酸（MUG）的Brolacin（CLED）琼脂和麦康凯琼脂组成。使用1022份尿液样本，将该产品与校准接种环接种CLED琼脂和血琼脂用于筛查尿液中尿路病原体的常规方法进行比较。还评估了用于鉴定大肠杆菌的MUG测试。
结果
与常规方法相比，Cult-Dip Plus的灵敏度、特异性、阳性预测值和阴性预测值分别为88.3%、98.0%、91.9%和97.1%。MUG测试正确鉴定了92%的大肠杆菌分离株，其灵敏度、特异性和阳性预测值分别为91.6%、95.2%和93.6%。
结论
Cult-Dip Plus似乎是检测尿路病原体的校准环法的替代方法。MUG测试可快速、可靠且廉价地鉴定大肠杆菌。
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