Butler W R, Thibert L, Kilburn J O
Mycobacteriology Laboratory, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1992 Oct;30(10):2698-704. doi: 10.1128/jcm.30.10.2698-2704.1992.
Strains of Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum, Mycobacterium xenopi, and Mycobacterium gordonae were identified by high-performance liquid chromatography (HPLC) analysis of mycolic acids as bromophenacyl esters. HPLC criteria were used to develop a flow chart identification scheme, which was evaluated in our laboratory with a set of 234 strains representing five species and a hitherto undescribed species. Correct identifications of M. gordonae and M. xenopi were easily made. Flow chart differentiation of M. avium, M. intracellulare, and M. scrofulaceum was done with 97.9, 97.5, and 89.2% accuracies, respectively. Independent evaluation of the flow chart at a separate laboratory demonstrated an overall identification accuracy of 97% for M. avium complex. Strains that have been described biochemically as being intermediate between M. avium-M. intracellulare and M. scrofulaceum were identified as one or the other of these known species. Strains which were negative with the species-specific radioactive probe for M. avium complex but which were positive with the nonradioactive SNAP X probe were usually identified as M. intracellulare and M. scrofulaceum but rarely as M. avium.
通过将分枝菌酸作为溴苯甲酰酯进行高效液相色谱（HPLC）分析，鉴定了鸟分枝杆菌、胞内分枝杆菌、瘰疬分枝杆菌、偶发分枝杆菌和戈登分枝杆菌菌株。利用HPLC标准制定了一个流程图鉴定方案，该方案在我们实验室用一组代表五个菌种和一个迄今未描述菌种的234株菌株进行了评估。戈登分枝杆菌和偶发分枝杆菌的正确鉴定很容易做出。鸟分枝杆菌、胞内分枝杆菌和瘰疬分枝杆菌的流程图鉴别准确率分别为97.9%、97.5%和89.2%。在另一个实验室对该流程图进行的独立评估表明，鸟分枝杆菌复合群的总体鉴定准确率为97%。在生物化学上被描述为介于鸟分枝杆菌-胞内分枝杆菌和瘰疬分枝杆菌之间的菌株被鉴定为这两个已知菌种中的一个。对鸟分枝杆菌复合群的种特异性放射性探针呈阴性但对非放射性SNAP X探针呈阳性的菌株通常被鉴定为胞内分枝杆菌和瘰疬分枝杆菌，但很少被鉴定为鸟分枝杆菌。
