Bashir M S, Morrison K, Wright D H, Jones D B
University Department of Pathology, South Block General Hospital, Southampton.
J Clin Pathol. 1992 Sep;45(9):776-80. doi: 10.1136/jcp.45.9.776.
To investigate the expression of mRNA transcripts containing exon A or B in lymphocyte cultures.
An in situ hybridisation technique, using synthetic, biotinylated oligonucleotide probes was deployed to allow the demonstration of exon A, exon B, or the normal hepatocyte message containing exon C.
Lymphocytes used the same alternative splicing technique as monocytes in the generation of their alpha-1 antitrypsin message. They also provided data on the frequency of exon A and B expression in cells from different subjects. Most circulating granulocytes failed to show the alpha-1 antitrypsin message, suggesting that this protein is synthesised in the marrow and represents a stored protein component in polymorph and circulating nuclear lymphocytes.
In situ hybridisation is a sensitive technique for the detection of individual gene exon use in cell populations. Lymphocytes show the same promoter use as that described for monocytes.
研究淋巴细胞培养物中含外显子A或B的mRNA转录本的表达情况。
采用合成的生物素化寡核苷酸探针的原位杂交技术，以显示外显子A、外显子B或含外显子C的正常肝细胞信息。
淋巴细胞在产生α-1抗胰蛋白酶信息时使用了与单核细胞相同的可变剪接技术。它们还提供了来自不同受试者细胞中外显子A和B表达频率的数据。大多数循环粒细胞未显示α-1抗胰蛋白酶信息，这表明该蛋白在骨髓中合成，是多形核细胞和循环核淋巴细胞中的一种储存蛋白成分。
原位杂交是检测细胞群体中单个基因外显子使用情况的灵敏技术。淋巴细胞显示出与单核细胞相同的启动子使用情况。
