Kalsheker N, Swanson T
Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff Royal Infirmary, United Kingdom.
Biochem Biophys Res Commun. 1990 Nov 15;172(3):1116-21. doi: 10.1016/0006-291x(90)91562-7.
Monocytes potentially express two mRNA species by alternative splicing of exons at the 5' end of the gene. During inflammation the plasma concentration of AAT can increase about three-fold. In a myelomonocytic cell line (U937), under basal conditions, two AAT mRNA species are produced. After stimulation by interleukin-6 (IL-6) one mRNA species is produced resulting from the exclusion of the second monocyte exon. These findings demonstrate a novel way in which the expression of mRNA can be modified in a single cell type.
单核细胞可能通过该基因5'端外显子的可变剪接表达两种mRNA。在炎症期间，α1抗胰蛋白酶（AAT）的血浆浓度可增加约三倍。在髓单核细胞系（U937）中，在基础条件下可产生两种AAT mRNA。在白细胞介素-6（IL-6）刺激后，由于第二个单核细胞外显子的排除，产生了一种mRNA。这些发现证明了一种新的方式，通过这种方式可以在单一细胞类型中修饰mRNA的表达。
