病毒感染细胞中溶酶体酶的激活及其与细胞病变效应的可能关系。
Activation of lysosomal enzymes in virus-infected cells and its possible relationship to cytopathic effects.
作者信息
ALLISON A C, SANDELIN K
出版信息
J Exp Med. 1963 Jun 1;117(6):879-87. doi: 10.1084/jem.117.6.879.
Abstract
Activities of the enzymes beta-glucuronidase, acid phosphatase, acid DNAase, acid RNAase, and acid protease have been measured in the lysosomal and supernatant fractions of mouse liver cells and monkey kidney cells before and after infection with mouse hepatitis virus and vaccinia virus, respectively. In the infected cells there was easily measurable release of lysosomal enzymes into the supernatant fraction. Evidence was presented that this is not an artefact of homogenization and precedes cell degeneration demonstrable histologically. It is suggested that release of lysosomal enzymes may explain some of the biochemical changes found in infected cells and may contribute to the cytopathic effects of some viruses.
摘要
分别在小鼠肝细胞和猴肾细胞感染小鼠肝炎病毒和痘苗病毒之前及之后，测定了溶酶体和上清液部分中β-葡萄糖醛酸酶、酸性磷酸酶、酸性脱氧核糖核酸酶、酸性核糖核酸酶和酸性蛋白酶的活性。在受感染的细胞中，溶酶体酶很容易检测到释放到上清液部分。有证据表明，这不是匀浆的人为现象，且在组织学上可证实的细胞变性之前就已发生。有人提出，溶酶体酶的释放可能解释了在受感染细胞中发现的一些生化变化，并可能导致某些病毒的细胞病变效应。
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