Suzuki Y, Yoshikawa K, Yokochi T
Department of Microbiology, Aichi Medical University, School of Medicine, Japan.
J Immunoassay. 1991;12(1):145-57. doi: 10.1080/01971529108055062.
An automated fluorometric assay using carboxyfluorescein diacetate (CFDA) has been applied for the sensitive and rapid detection of natural killer (NK) activity. The lysis of target cells by NK cells was quantified by measuring the amount of CFDA released into the supernatant of culture wells with the aid of an automated microfluorometer. Both sensitivity and specificity of the presented method were higher than the 51Cr release assay. Moreover, the detection of human NK activity against K562 target cells required only 2 hrs, compared to 4 hrs in the standard 51Cr release assay.
一种使用羧基荧光素二乙酸酯（CFDA）的自动荧光测定法已被用于灵敏且快速地检测自然杀伤（NK）活性。通过借助自动微量荧光计测量释放到培养孔上清液中的CFDA量，对NK细胞介导的靶细胞裂解进行定量。所提出方法的灵敏度和特异性均高于51Cr释放测定法。此外，检测人NK细胞对K562靶细胞的活性仅需2小时，而标准51Cr释放测定法则需要4小时。
