Täuber M G, Sachdeva M, Kennedy S L, Loetscher H, Lesslauer W
Microbial Pathogenesis Unit, San Francisco General Hospital, CA.
J Infect Dis. 1992 Nov;166(5):1045-50. doi: 10.1093/infdis/166.5.1045.
To identify neurotoxic factors in meningitis, a neuronal cell line (HN33.1) was exposed to cerebrospinal fluid (CSF) obtained from rabbits with pneumococcal meningitis or Escherichia coli meningitis or 2 h and 6 h after meningitis was induced by proinflammatory bacterial products (pneumococcal cell walls, endotoxin). CSF from all types of meningitis induced similar degrees of cytotoxicity. When a soluble tumor necrosis factor (TNF) receptor that completely blocked TNF-mediated toxicity at 10(-7) M was used, all toxicity in meningitis caused by E. coli, endotoxin, or pneumococcal cell wall administration (2 h afterwards) was mediated by TNF. In contrast, CSF from animals with meningitis caused by live pneumococci or pneumococcal cell wall injection (6 h afterwards) retained cytotoxicity in the presence of the TNF receptor. Thus, in established pneumococcal meningitis, but not in the other forms of meningitis, TNF is not the only component toxic in this neuronal cell line.
为了鉴定脑膜炎中的神经毒性因子，将一种神经元细胞系（HN33.1）暴露于从患有肺炎球菌性脑膜炎或大肠杆菌性脑膜炎的兔子获取的脑脊液中，或暴露于由促炎性细菌产物（肺炎球菌细胞壁、内毒素）诱导脑膜炎2小时和6小时后的脑脊液中。来自所有类型脑膜炎的脑脊液诱导出相似程度的细胞毒性。当使用在10^(-7) M时能完全阻断TNF介导毒性的可溶性肿瘤坏死因子（TNF）受体时，由大肠杆菌、内毒素或肺炎球菌细胞壁给药（2小时后）引起的脑膜炎中的所有毒性均由TNF介导。相比之下，来自因活肺炎球菌或肺炎球菌细胞壁注射（6小时后）导致脑膜炎的动物的脑脊液在TNF受体存在的情况下仍保留细胞毒性。因此，在已形成的肺炎球菌性脑膜炎中，但在其他形式的脑膜炎中并非如此，TNF不是该神经元细胞系中唯一具有毒性的成分。
