Tuomanen E, Tomasz A, Hengstler B, Zak O
J Infect Dis. 1985 Mar;151(3):535-40. doi: 10.1093/infdis/151.3.535.
The relative contribution of bacterial components to the induction of inflammation during Streptococcus pneumoniae meningitis is unknown. Several strains of pneumococci with differences in cell surface characteristics (capsule or cell wall) were compared for the effect on the inflammatory response evoked during infection of the cerebrospinal fluid (CSF) in vivo. The presence of bacterial capsular polysaccharide was not necessary for bacterial growth in CSF in vivo but correlated with greater CSF bacterial density. CSF inflammatory changes began to appear when the bacterial concentration exceeded 10(5) cfu/ml, regardless of the pneumococcal strain. CSF inflammatory changes could be invoked by cisternal instillation of 10(5)-10(6) cell equivalents of whole, heat-killed unencapsulated strains or their isolated cell walls but not by similar concentrations of heat-killed encapsulated strains or isolated capsular polysaccharide. Hypoglycorrhachia was observed only during inflammation caused by live bacteria. The inflammatory response characteristic of naturally acquired pneumococcal meningitis can be reproduced by challenge with both encapsulated and uncapsulated bacteria. The bacterial cell wall appears to be the most potent pneumococcal surface component in inducing CSF inflammation.
肺炎链球菌脑膜炎期间细菌成分对炎症诱导的相对贡献尚不清楚。比较了几种细胞表面特征（荚膜或细胞壁）不同的肺炎球菌菌株，以观察其对体内脑脊液（CSF）感染过程中引发的炎症反应的影响。细菌荚膜多糖的存在对于肺炎球菌在体内脑脊液中的生长并非必需，但与脑脊液中更高的细菌密度相关。当细菌浓度超过10⁵cfu/ml时，无论肺炎球菌菌株如何，脑脊液炎症变化开始出现。通过脑池内注入10⁵ - 10⁶个细胞当量的完整、热灭活的无荚膜菌株或其分离的细胞壁可引发脑脊液炎症变化，但相似浓度的热灭活荚膜菌株或分离的荚膜多糖则不能。仅在由活细菌引起的炎症期间观察到脑脊液糖含量降低。天然获得性肺炎球菌脑膜炎的炎症反应特征可通过用有荚膜和无荚膜细菌攻击来重现。细菌细胞壁似乎是诱导脑脊液炎症的最有效的肺炎球菌表面成分。
