COLE A, LANGLEY R
Biophys J. 1963 May;3(3):189-97. doi: 10.1016/s0006-3495(63)86815-0.
Hydrated T2 bacteriophage were irradiated with 0.75 to 90 kev electron beams. A thin foil isolated the sample chamber from the electron gun source. Survival (plaque formation) was observed. Apparent cross-sections and D(37) doses were determined. The maximum cross-section of about 5 x 10(-3) mu(2) is roughly equal to the cross-sectional area presented by the phage core. As beam energy was increased the average D(37) dose first attained a minimum value of about 23 kr for 1 kev electrons (which penetrate the relatively inert protein coat) after which the average D(37) dose rose with beam energy to a maximum value of about 50 kr for fully penetrating beams. These dependencies suggest that the radiosensitive structure exists as a peripheral shell rather than a uniformly sensitive core. A tentative model for the phage structure, based on this and other evidence, is presented.
用0.75至90千电子伏特的电子束照射水合T2噬菌体。一片薄箔将样品室与电子枪源隔开。观察存活情况（噬菌斑形成）。确定了表观横截面和D(37)剂量。约5×10⁻³微米²的最大横截面大致等于噬菌体核心呈现的横截面积。随着束流能量增加，平均D(37)剂量首先在1千电子伏特电子（能穿透相对惰性的蛋白质外壳）时达到约23千伦琴的最小值，之后平均D(37)剂量随着束流能量增加，对于完全穿透的束流达到约50千伦琴的最大值。这些相关性表明，放射敏感结构以周边壳层形式存在，而非均匀敏感的核心。基于此及其他证据，提出了噬菌体结构的初步模型。
