Funk F, Person S, Bockrath R C
Biophys J. 1968 Sep;8(9):1037-50. doi: 10.1016/S0006-3495(68)86537-3.
Coliphage T4 was used as a model system to study the mechanism of biological inactivation produced by tritium decay. Experimentally, tritiated precursors were incorporated into phage DNA (thymidine-(3)H) or into phage protein ((3)H-amino acids). The ratio of killing efficiencies for decays originating in phage DNA to those originating in phage protein was 2.6. Inactivation by decays from labeled amino acids was assumed to occur exclusively from beta-particle irradiation of phage DNA. If decays originating in DNA are due solely to irradiation of DNA, then the killing efficiencies reflect the energy transfer paths in phage DNA for decays originating in phage DNA and in the protein coat. The energy transfer paths were determined for the two cases with the help of a computer and found to be very nearly equal to the experimentally determined ratio (2.6). The killing efficiencies for decays originating in phage DNA were 0.12 and for decays originating in protein 0.046.
以大肠杆菌噬菌体T4作为模型系统来研究氚衰变产生生物灭活的机制。在实验中，将氚标记的前体掺入噬菌体DNA（胸腺嘧啶 - ³H）或噬菌体蛋白质（³H - 氨基酸）中。源自噬菌体DNA的衰变与源自噬菌体蛋白质的衰变的杀伤效率之比为2.6。假定来自标记氨基酸的衰变导致的灭活完全是由于噬菌体DNA受到β粒子辐照。如果源自DNA的衰变仅归因于DNA的辐照，那么杀伤效率反映了噬菌体DNA中源自噬菌体DNA和蛋白质外壳的衰变的能量转移路径。借助计算机确定了这两种情况下的能量转移路径，发现其非常接近实验测定的比率（2.6）。源自噬菌体DNA的衰变的杀伤效率为0.12，源自蛋白质的衰变的杀伤效率为0.046。
