DISINGER B L, WILSON J B
J Bacteriol. 1962 Nov;84(5):911-5. doi: 10.1128/JB.84.5.911-915.1962.
Dasinger, B. L. (University of Wisconsin, Madison) and J. B. Wilson. Glutamate metabolism in Brucella abortus strains of low and high virulence. J. Bacteriol. 84:911-915. 1962.-Brucella abortus strains of low virulence oxidize glutamate at a high rate, whereas strains of high virulence oxidize glutamate at a relatively low rate. Results indicated that this observation was not related to differences in pathway of glutamate oxidation or to differences in total enzyme activity. Permeability studies showed that the maximal rates of glutamate accumulation were 2.8 mumoles per 2 min per g (wet wt) for a strain of high virulence and 6.2 mumoles per 2 min per g for a strain of low virulence, but equal intracellular steady-state concentrations were attained by both types of strains. Evidence is presented which suggests that the site of glutamate oxidation is separate from the pool of glutamate being measured. Unequal rates of permeability at these sites could be the reason for the differences in rate of glutamate oxidation.
达辛格，B. L.（威斯康星大学麦迪逊分校）和J. B. 威尔逊。低毒力和高毒力流产布鲁氏菌菌株中的谷氨酸代谢。《细菌学杂志》84:911 - 915。1962年。——低毒力流产布鲁氏菌菌株以高速率氧化谷氨酸，而高毒力菌株氧化谷氨酸的速率相对较低。结果表明，这一观察结果与谷氨酸氧化途径的差异或总酶活性的差异无关。通透性研究表明，高毒力菌株每2分钟每克（湿重）谷氨酸积累的最大速率为2.8微摩尔，低毒力菌株为每2分钟每克6.2微摩尔，但两种类型的菌株都达到了相等的细胞内稳态浓度。有证据表明，谷氨酸氧化的部位与所测量的谷氨酸池是分开的。这些部位通透性速率的不平等可能是谷氨酸氧化速率差异的原因。
