Ionescu J, Tapu V, Eskenasy A
Morphol Embryol (Bucur). 1977 Jan-Mar;23(1):77-82.
Three experimental fibrosing granulomatous processes of the lung (pulmonary granulomas induced by complete Freund adjuvant, viral pneumonitis induced by the A2 influenza virus, and tuberculosis induced in animals exposed to tobacco smoke) were produced in rabbits and the results confronted in view of establishing some relationships between mesenchymal cell accumulations, reticulin fibrillogenesis, and the enzymic activities of proline-oxidase (PO) and of hydroxyproline-2-epimerase (HEP), enzymes intervening in the control of hydroxyproline incorporation in the procollagen molecule. Histopathologic, histochemical and histoenzymic methods, quantification of cells and fibrils, statistical analyses, including the regression lines method, were used. The confrontation of the three processes made obvious that the cell accumulation invariably preceded the fibrillogenetic process, that the immune nature of the process was accompanied by larger cell accumulations than the non-immune one, that between cell accumulations and fibrillogenesis there existed highly significant correlations, and that the increase of the enzymic activities of proline-oxidase and of hydroxyproline-2-epimerase always accompanied these tissular changes, being topographically coincident with them (interlobular areas, alveolar walls).
在兔身上诱发了三种实验性肺纤维化肉芽肿病变（由完全弗氏佐剂诱导的肺肉芽肿、由A2流感病毒诱发的病毒性肺炎以及在接触烟草烟雾的动物身上诱发的结核病），并对结果进行对比，以确定间充质细胞聚集、网状纤维形成以及脯氨酸氧化酶（PO）和羟脯氨酸-2-差向异构酶（HEP）的酶活性之间的某些关系，这些酶参与前胶原分子中羟脯氨酸掺入的控制。采用了组织病理学、组织化学和组织酶学方法、细胞和纤维的定量分析以及包括回归线法在内的统计分析。对这三种病变的对比表明，细胞聚集总是先于纤维形成过程，免疫性病变比非免疫性病变伴随有更大的细胞聚集，细胞聚集与纤维形成之间存在高度显著的相关性，脯氨酸氧化酶和羟脯氨酸-2-差向异构酶的酶活性增加总是伴随着这些组织学变化，且在地形上与它们一致（小叶间区域、肺泡壁）。
