KIBLER R F, BARNES A E
J Exp Med. 1962 Dec 1;116(6):807-25. doi: 10.1084/jem.116.6.807.
A water-soluble protein fraction of nervous tissue was prepared by extraction of rabbit spinal cord with sodium citrate at pH 4.3. Characterization by nitrogen content and paper electrophoresis showed it to be a mixture of basic proteins. This extract demonstrated encephalitogenic activity when injected into rabbits. The most suitable technique for the measurement of serum antibody to the rabbit cord antigen proved to be the precipitation of antigen-antibody complexes by 40 per cent saturated ammonium sulfate. Antibody could not be demonstrated by the techniques of complement fixation, quantitative precipitation, and Ouchterlony plates. The early appearance of circulating antibody occurred almost exclusively in rabbits that subsequently developed EAE. Specificity of the antibodies for nervous tissue was demonstrated by appropriate blocking and adsorption experiments.
通过在pH 4.3条件下用柠檬酸钠提取兔脊髓,制备了神经组织的水溶性蛋白质部分。通过氮含量和纸电泳进行表征,结果表明它是碱性蛋白质的混合物。将该提取物注射到兔子体内时表现出致脑炎活性。事实证明,测量针对兔脊髓抗原的血清抗体的最合适技术是用40%饱和硫酸铵沉淀抗原-抗体复合物。补体结合、定量沉淀和双向免疫扩散平板技术均无法检测到抗体。循环抗体的早期出现几乎只发生在随后发生实验性变态反应性脑脊髓炎(EAE)的兔子身上。通过适当的阻断和吸附实验证明了抗体对神经组织的特异性。