Juto P, Holm S
Department of Clinical Virology, University Hospital, Umeå, Sweden.
J Pediatr Gastroenterol Nutr. 1992 Aug;15(2):159-62. doi: 10.1097/00005176-199208000-00010.
The presence of gliadin-specific IgA and IgG antibodies in colostrum and serum of 140 newly delivered mothers was assessed by enzyme-linked immunosorbent assay. In addition, cow's milk protein (CMP)-specific IgA was determined in the colostrum samples. From 14 of the mothers longitudinal milk samples were obtained after 1 and 2 months of lactation and from 12 mothers after 3 months. Gliadin-specific IgA was found in 97.1% and gliadin-specific IgG in 9.3% of the colostrum samples. Gliadin-specific IgA was detected in mature samples but at significantly lower levels after 1, 2, and 3 months of lactation (p less than 0.01) as compared with colostrum. Gliadin-specific IgA was found in 2.8% of the serum samples and gliadin-specific IgG in 40%; however, the levels of both isotypes were low. CMP-specific IgA was found in 78.1% of the colostrum samples. It is concluded that IgA antibodies to two common food proteins are frequently found in human milk and that food-specific IgA present in milk may play a role in adapting the infant's immune reactions to food antigens in the gut.
采用酶联免疫吸附测定法评估了140名刚分娩母亲初乳和血清中麦醇溶蛋白特异性IgA和IgG抗体的存在情况。此外，还测定了初乳样本中牛乳蛋白（CMP）特异性IgA。从14名母亲处采集了产后1个月和2个月的纵向乳汁样本，从12名母亲处采集了产后3个月的乳汁样本。在97.1%的初乳样本中发现了麦醇溶蛋白特异性IgA，在9.3%的初乳样本中发现了麦醇溶蛋白特异性IgG。在成熟乳汁样本中检测到了麦醇溶蛋白特异性IgA，但与初乳相比，产后1、2和3个月时其水平显著降低（p<0.01）。在2.8%的血清样本中发现了麦醇溶蛋白特异性IgA，在40%的血清样本中发现了麦醇溶蛋白特异性IgG；然而，两种同种型的水平都很低。在78.1%的初乳样本中发现了CMP特异性IgA。结论是，人乳中经常发现针对两种常见食物蛋白的IgA抗体，且乳汁中存在的食物特异性IgA可能在使婴儿免疫反应适应肠道中的食物抗原方面发挥作用。
