Medical Research Institute La Fe, Unidad de Enfermedad Celíaca e Inmunopatología Digestiva, Torre A, 6º Planta. Avda. Fernando Abril Martorell 106, 46026, Valencia, Spain.
Department of Paediatrics, Leiden University Medical Centre, Leiden, The Netherlands.
Eur J Nutr. 2018 Aug;57(5):1947-1955. doi: 10.1007/s00394-017-1476-1. Epub 2017 May 29.
To analyze the presence of total IgA and anti-gliadin antibodies (AGA) in BM from CD mothers who follow a gluten-free diet (GFD) and from mothers on a normal gluten-containing diet (ND).
218 samples of mature milk were obtained at different months of lactation (1-6) from 83 mothers (2 or more samples per mother) from Italy (Naples), The Netherlands (Leiden) and Spain (Madrid, Valencia and Reus): 42 CD mothers on GFD for more than 2 years and 41 non-CD mothers on a ND. Whey samples were analyzed for AGA-IgA by an indirect homemade ELISA and for total IgA (g/L) by a commercial ELISA kit.
AGA-IgA was detected in BM, both in mothers on a GFD and mothers on a ND. AGA-IgA levels in both groups of mothers, CD and non-CD, show the same trend towards decreasing slightly along the months of lactation (p = 0.91). A different trend is observed for total IgA levels, decreasing markedly in CD mothers from the first month of lactation onwards but remaining stable in non-CD mothers (p = 0.048). A statistically significant association was found between the means of total IgA and AGA-IgA (p < 0.001).
AGA-IgA is present in BM from mothers on a ND as well as in BM from mothers who had been on a GFD for years. This reflects the existence of a long-lasting immunological memory independent of the mother's diet. If the presence of these antibodies has any role in promoting the acquisition of gluten tolerance in the infant, our study shows that children of CD mothers would be on equal conditions as children of non-CD mothers.
分析遵循无麸质饮食（GFD）的 CD 母亲和正常含麸质饮食（ND）的母亲的 BM 中总 IgA 和抗麦胶蛋白抗体（AGA）的存在情况。
从意大利（那不勒斯）、荷兰（莱顿）和西班牙（马德里、瓦伦西亚和雷乌斯）的 83 位母亲（每位母亲 2 个或更多样本）中获得了不同哺乳期（1-6 个月）的 218 份成熟乳样本：42 位 CD 母亲遵循 GFD 超过 2 年，41 位非 CD 母亲遵循 ND。通过间接自制 ELISA 分析乳清样本中的 AGA-IgA，通过商业 ELISA 试剂盒分析总 IgA（g/L）。
在 GFD 和 ND 的母亲的 BM 中均检测到 AGA-IgA。CD 和非 CD 母亲两组的 AGA-IgA 水平均呈逐月略有下降的趋势（p=0.91）。总 IgA 水平的趋势则不同，CD 母亲从哺乳期第一个月开始明显下降，但非 CD 母亲保持稳定（p=0.048）。总 IgA 和 AGA-IgA 的平均值之间存在统计学显著关联（p<0.001）。
ND 母亲的 BM 以及已经遵循 GFD 多年的母亲的 BM 中均存在 AGA-IgA。这反映了一种独立于母亲饮食的持久免疫记忆的存在。如果这些抗体的存在对促进婴儿获得麸质耐受有任何作用，我们的研究表明，CD 母亲的孩子与非 CD 母亲的孩子处于同等条件。
