KOSUNEN T U, WAKSMAN B H, FLAX M H, TIHEN W S
Immunology. 1963 May;6(3):276-90.
In delayed skin reactions to PPD and BSA in the rat and to old tuberculin and diphtheria toxoid in the guinea-pig, intravenous injection of tritium-labelled thymidine shortly before skin testing produced labelling of a high proportion of the cells entering the perivascular infiltrates 12–18 hours later. Administration of thymidine at various times after skin testing gave immediate labelling of 5–10 per cent of cells in the infiltrates. It was concluded that the majority of the cells in the infiltrates are haematogenous, that they are synthesizing DNA before entering the lesion, and that many do so in the lesion itself. The principal cell type labelled in both experiments resembled a medium-sized lymphocyte of the lymphoid tissues or large lymphocyte of the blood. There was evidence that over several hours many of these became typical histiocytes. In non-specific reactions, a similar pattern of labelling was observed but the total number of infiltrating cells was very small.
在大鼠对结核菌素纯蛋白衍生物（PPD）和牛血清白蛋白（BSA）以及豚鼠对旧结核菌素和白喉类毒素的迟发性皮肤反应中，在皮肤试验前不久静脉注射氚标记的胸腺嘧啶核苷，会使12 - 18小时后进入血管周围浸润区的细胞中有很大比例被标记。在皮肤试验后的不同时间给予胸腺嘧啶核苷，会使浸润区5 - 10%的细胞立即被标记。得出的结论是，浸润区的大多数细胞是血源性的，它们在进入病变部位之前正在合成DNA，而且许多细胞在病变部位本身也在进行DNA合成。在两个实验中被标记的主要细胞类型类似于淋巴组织中的中等大小淋巴细胞或血液中的大淋巴细胞。有证据表明，在几个小时内，其中许多细胞变成了典型的组织细胞。在非特异性反应中，观察到了类似的标记模式，但浸润细胞的总数非常少。
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