结核菌素反应中抗原定位的电子显微镜观察

Immunology. 1965 Jan;8(1):81-7.

The tuberculin reaction was examined in the electron microscope using as antigen PPD conjugated to ferritin. The ferritin marker was rapidly localized within macrophages prior to the time of significant lymphocyte accumulation. Evidence of degenerative changes in macrophages was seen. Uptake of marker by lymphocytes was not noted. Appropriate control experiments indicated that the uptake of antigen by macrophages and subsequent macrophage degeneration were not dependent on previous sensitization. The accumulation of lymphocytes without apparent contact with unaltered antigen was interpreted as evidence that lymphocyte accumulation was a consequence of the prior interaction between antigen and macrophages.

使用与铁蛋白结合的结核菌素纯蛋白衍生物（PPD）作为抗原，在电子显微镜下检测结核菌素反应。在大量淋巴细胞聚集之前，铁蛋白标记物迅速定位于巨噬细胞内。可见巨噬细胞发生退行性变化的证据。未观察到淋巴细胞摄取标记物。适当的对照实验表明，巨噬细胞对抗原的摄取及随后的巨噬细胞变性并不依赖于先前的致敏作用。淋巴细胞在未与未改变的抗原明显接触的情况下聚集，这被解释为淋巴细胞聚集是抗原与巨噬细胞先前相互作用的结果的证据。