Okazaki J, Kamada A, Gonda Y, Sakaki T
First Department of Prosthodontics, Osaka Dental University, Japan.
J Periodontal Res. 1992 Sep;27(5):484-8. doi: 10.1111/j.1600-0765.1992.tb01821.x.
Glycosaminoglycan (GAG) was extracted from monkey periodontium, consisting of gingiva, periodontal ligament, alveolar bone and cementum, and from dental pulp and dentin by digestion with Pronase E. Unsaturated disaccharide isomers formed by chondroitinase AC digestion from chondroitin sulfate were labeled with dansylhydrazine and analyzed by high-performance liquid chromatography. These tissues showed different molar ratios of the unsaturated chondroitin sulfate disaccharides. The ratio of delta Di-4S to delta Di-6S was lowest in the dental pulp, followed by the gingiva, periodontal ligament, dentin, alveolar bone, and cementum, in that order. It was greater in the calcified than in the uncalcified tissues.
通过用链霉蛋白酶E消化，从猴牙周组织（包括牙龈、牙周韧带、牙槽骨和牙骨质）以及牙髓和牙本质中提取糖胺聚糖（GAG）。用丹磺酰肼标记硫酸软骨素经软骨素酶AC消化形成的不饱和二糖异构体，并通过高效液相色谱法进行分析。这些组织显示出不同的硫酸软骨素不饱和二糖的摩尔比。牙髓中δDi-4S与δDi-6S的比值最低，其次依次为牙龈、牙周韧带、牙本质、牙槽骨和牙骨质。钙化组织中的该比值高于未钙化组织。
