Okazaki J, Gonda Y, Kamada A, Fujita A, Sakaki T
First Department of Prosthodontics, Osaka Dental University, Japan.
J Osaka Dent Univ. 1993 Oct;27(2):101-6.
We investigated the chondroitin sulfate in human periodontal samples (gingiva, periodontal ligament, cementum and alveolar bone) collected for orthodontic reasons. Glycosaminoglycans (GAGs) were extracted from the periodontium by enzyme digestion, and unsaturated disaccharide isomers of chondroitin sulfate were obtained by chondroitinase ACII and hyaluronidase digestion. The isomers were analyzed by high-performance liquid chromatography. Chondroitin sulfate was found in all four types of periodontal tissue; its unsaturated disaccharide isomers consisted in delta Di-0S, delta Di-6S, delta Di-4S, delta Di-diSE and delta Di-triS. These four types of periodontal tissue showed different molar ratios of the unsaturated disaccharides. The ratio of delta Di-4S to delta Di-6S was greater in the calcified than in the uncalcified tissue.
我们研究了因正畸原因收集的人类牙周样本（牙龈、牙周韧带、牙骨质和牙槽骨）中的硫酸软骨素。通过酶消化从牙周组织中提取糖胺聚糖（GAGs），并通过软骨素酶ACII和透明质酸酶消化获得硫酸软骨素的不饱和二糖异构体。通过高效液相色谱法对这些异构体进行分析。在所有四种类型的牙周组织中均发现了硫酸软骨素；其不饱和二糖异构体包括δDi-0S、δDi-6S、δDi-4S、δDi-diSE和δDi-triS。这四种类型的牙周组织显示出不饱和二糖的不同摩尔比。钙化组织中δDi-4S与δDi-6S的比值高于未钙化组织。
