Okazaki J, Kamada A, Gonda Y, Sakaki T, Embery G
First Department of Prosthodontics, Osaka Dental University, Japan.
Oral Dis. 1996 Sep;2(3):224-7. doi: 10.1111/j.1601-0825.1996.tb00228.x.
Tests have been carried out to assess the level of unsaturated disaccharide isomers obtained from chondroitin sulphate in whole saliva, which contains chondroitin sulphate derived from gingival crevicular fluid (GCF).
Whole saliva was collected from periodontally diseased subjects (PDS), clinically healthy subjects (CHS) and edentulous subjects (ES). Glycosaminoglycans (GAG) were liberated by digestion with Pronase E, and precipitated with cetylpyridinium chloride and ethanol. The unsaturated disaccharides obtained by chondroitinase ACII digestion of the liberated GAG were analysed by high-performance liquid chromatography. The unsaturated disaccharides included delta Di-0S, delta Di-6S and delta Di-4S.
Analysis of data indicated that delta Di-0S, delta Di-6S and delta Di-4S were found in all PDS samples. The amount (ng ml-1 collected whole saliva) of delta Di-0S, delta Di-6S and delta Di-4S (P < 0.01) indicated significant differences between CHS and PDS whole saliva samples. The quantities of delta Di-0S and delta Di-4S (P < 0.01) indicated significant differences between PDS and ES whole saliva. The amount of delta Di-0S (P < .05) and delta Di-6S (P < 0.01) also indicated significant differences between CHS and ES whole saliva. These results indicate that chondroitin sulphate in PDS and CHS whole saliva is representative of that previously reported in gingival crevicular fluid and so provides a useful and alternative means of assessing the role of GAG as indicators of periodontal disease.
已开展相关测试，以评估全唾液中从硫酸软骨素获得的不饱和二糖异构体水平，全唾液中含有源自龈沟液（GCF）的硫酸软骨素。
从牙周病患者（PDS）、临床健康受试者（CHS）和无牙受试者（ES）中收集全唾液。通过链霉蛋白酶E消化释放糖胺聚糖（GAG），并用十六烷基氯化吡啶和乙醇沉淀。通过高效液相色谱分析经软骨素酶ACII消化释放的GAG所获得的不饱和二糖。不饱和二糖包括δDi-0S、δDi-6S和δDi-4S。
数据分析表明，在所有PDS样本中均发现了δDi-0S、δDi-6S和δDi-4S。δDi-0S、δDi-6S和δDi-4S的量（每毫升收集的全唾液中的纳克数）（P<0.01）表明CHS和PDS全唾液样本之间存在显著差异。δDi-0S和δDi-4S的量（P<0.01）表明PDS和ES全唾液之间存在显著差异。δDi-0S的量（P<0.05）和δDi-6S的量（P<0.01）也表明CHS和ES全唾液之间存在显著差异。这些结果表明，PDS和CHS全唾液中的硫酸软骨素代表了先前在龈沟液中报道的硫酸软骨素，因此提供了一种有用的替代方法来评估GAG作为牙周疾病指标的作用。
