Imai K, Aimoto T, Sato M, Kimura R
Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Yakugaku Zasshi. 1992 Apr;112(4):253-8. doi: 10.1248/yakushi1947.112.4_253.
The distribution and the anti-lipid peroxidative effects of porphyrins in the hepatic subcellular fractions in rats were studied after intravenous administration of protoporphyrin (PP). PP and/or PP-derived porphyrins were mainly distributed in the membrane-containing fractions of 600 x g-, 10,000 x g- and 100,000 x g-sediment from the liver homogenates of rats receiving a 20 mg/kg dose of PP. The lipid peroxidation induced by L-ascorbic acid in the fractions of 600 x g-, 10,000 x g- and 100,000 x g-sediment from the PP-treated rats was suppressed during 1-168 h, 1-168 h and 12-168 h, respectively, after the PP treatment. The suppression of the peroxidation in the liver mitochondria from the PP-treated rats was further enhanced by the addition of the hepatic cytosol from the PP-treated rats. The extent of the suppression by the addition of the cytosol from the PP-treated rats at 24 h after the PP administration was greater than those at 0 and 168 h after the PP administration. These results indicate that PP and/or PP-derived porphyrins distributed in the liver still exert antioxidative actions and that there might exist some unknown factors enhancing the actions in the hepatic cytosol.
在给大鼠静脉注射原卟啉（PP）后，研究了卟啉在大鼠肝脏亚细胞组分中的分布及其抗脂质过氧化作用。给予20mg/kg剂量PP的大鼠肝脏匀浆中，PP和/或PP衍生的卟啉主要分布在600×g、10,000×g和100,000×g沉淀的含膜组分中。PP处理大鼠的600×g、10,000×g和100,000×g沉淀组分中，L-抗坏血酸诱导的脂质过氧化在PP处理后的1-168小时、1-168小时和12-168小时分别受到抑制。添加PP处理大鼠的肝细胞溶胶可进一步增强PP处理大鼠肝脏线粒体中的过氧化抑制作用。PP给药后24小时添加PP处理大鼠的细胞溶胶的抑制程度大于PP给药后0小时和168小时的抑制程度。这些结果表明，分布在肝脏中的PP和/或PP衍生的卟啉仍发挥抗氧化作用，并且在肝细胞溶胶中可能存在一些未知因素增强了这些作用。
