Kerr J B
Department of Anatomy, Monash University, Clayton, Melbourne, Victoria, Australia.
J Reprod Fertil. 1992 Aug;95(3):825-30. doi: 10.1530/jrf.0.0950825.
The occurrence of degenerating germ cells in the cycle of the seminiferous epithelium was measured in testicular tissues from eight normal adult rats. Testes were perfusion fixed, embedded in epoxy resin and, after sectioning a total of 180 randomly selected blocks at 1 microns, stained sections were examined by light microscopy; all cross-sectioned seminiferous tubules were categorized into one of 14 stages of the spermatogenic cycle. The number of degenerating cells per tubule was recorded in 2103 tubules. Degenerating germ cells were not detected at stages II-VI, and only rarely at stage VII (n = 366 tubules) in which one primary spermatocyte and one step 19 spermatid degenerated. All other stages exhibited a greater incidence of degenerative germ cells, particularly at stage XIV where, on average, the frequency of degenerating cells per round seminiferous tubule was about 40 times greater than at stage VII. The results indicated that, in the normal adult rat testis, the germ cells are least at risk of degeneration as they pass through stage VII.
在八只正常成年大鼠的睾丸组织中，测定了生精上皮周期中退化生殖细胞的发生率。睾丸经灌注固定，包埋于环氧树脂中，在将总共180个随机选取的组织块切成1微米厚的切片后，通过光学显微镜检查染色切片；所有横切的生精小管被归类为生精周期的14个阶段之一。在2103个小管中记录了每个小管中退化细胞的数量。在II-VI阶段未检测到退化生殖细胞，在VII阶段也很少检测到（n = 366个小管），其中有一个初级精母细胞和一个19期精子细胞退化。所有其他阶段退化生殖细胞的发生率更高，特别是在XIV阶段，平均而言，每圆形生精小管中退化细胞频率比VII阶段大约高40倍。结果表明，在正常成年大鼠睾丸中，生殖细胞在通过VII阶段时退化风险最低。
