Kim H L, Ray A C, Calhoun M C
Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station 77843.
J Toxicol Environ Health. 1992 Oct;37(2):341-7. doi: 10.1080/15287399209531674.
Metabolites of ethoxyquin (EQ, 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline) in the urine of sheep and rats were separated and identified by gas chromatography-mass spectrometry (GC-MS). Sheep were given diets containing EQ or EQ.HCl (0.5% of total diet) and urine samples were collected for the first 24 h and for another 24-h period after 12 d of feeding. Rats were given EQ/corn oil (0.08 g EQ/d/rat) orally for 7 d and urine samples were collected at ambient temperature for a 24-h period following 6 d of dosing. The urine samples were extracted with ethyl acetate at pH 5, and the concentrated extracts were analyzed by GC-MS. Ethoxyquin was identified in all sheep urine samples collected during the first 24 h of feeding, and EQ and hydroxylated EQ were identified in all urine samples collected after 12 d of feeding. In contrast, EQ, hydroxylated EQ, and dihydroxylated EQ were identified in urine collected from rats fed EQ for 7 d.
通过气相色谱 - 质谱联用仪（GC-MS）对绵羊和大鼠尿液中乙氧喹（EQ，1,2 - 二氢 - 6 - 乙氧基 - 2,2,4 - 三甲基喹啉）的代谢产物进行了分离和鉴定。给绵羊喂食含EQ或EQ·HCl（占总日粮的0.5%）的日粮，在喂食12天后的头24小时以及随后的另一个24小时期间收集尿液样本。给大鼠口服EQ/玉米油（0.08 g EQ/天/只大鼠），持续7天，并在给药6天后于室温下收集24小时的尿液样本。尿液样本在pH 5的条件下用乙酸乙酯萃取，浓缩提取物通过GC-MS进行分析。在喂食的头24小时收集的所有绵羊尿液样本中均鉴定出乙氧喹，在喂食12天后收集的所有尿液样本中鉴定出EQ和羟基化EQ。相比之下，在喂食EQ 7天的大鼠收集的尿液中鉴定出EQ、羟基化EQ和二羟基化EQ。
