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厌氧生长对表皮葡萄球菌硝酸盐还原的影响

EFFECT OF ANAEROBIC GROWTH ON NITRATE REDUCTION BY STAPHYLOCOCCUS EPIDERMIDIS.

作者信息

JACOBS N J, JOHANTGES J, DEIBEL R H

出版信息

J Bacteriol. 1963 Apr;85(4):782-7. doi: 10.1128/jb.85.4.782-787.1963.

DOI:10.1128/jb.85.4.782-787.1963
PMID:14044943
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC278225/
Abstract

Jacobs, N. J. (American Meat Institute Foundation, Chicago, Ill.), J. Johantges, and R. H. Deibel. Effect of anaerobic growth on nitrate reduction by Staphylococcus epidermidis. J. Bacteriol. 85:782-787. 1963.-In anaerobic cultures, a strain of Staphylococcus epidermidis failed to reduce nitrate, although nitrate was reduced in stationary cultures grown in the presence of air. Resting suspensions of cells grown in air reduced nitrate. Although suspensions of cells grown anaerobically did not reduce nitrate, the nitrate reductase enzyme was shown to be present. When the artificial electron carrier, benzyl viologen, was added to suspensions of cells grown anaerobically, nitrate was reduced, indicating that an electron carrier required for nitrate reduction was missing. The addition of hemin to the growth medium enabled anaerobic cultures to reduce nitrate.

摘要

雅各布斯,N. J.(美国肉类研究所基金会,伊利诺伊州芝加哥),J. 约翰特格斯,以及R. H. 戴贝尔。厌氧生长对表皮葡萄球菌硝酸盐还原的影响。《细菌学杂志》85:782 - 787。1963年。——在厌氧培养中,一株表皮葡萄球菌无法还原硝酸盐,尽管在有空气存在下生长的静止培养物中硝酸盐会被还原。在空气中生长的细胞静置悬浮液能还原硝酸盐。虽然厌氧生长的细胞悬浮液不能还原硝酸盐,但显示存在硝酸盐还原酶。当向厌氧生长的细胞悬浮液中添加人工电子载体苄基紫精时,硝酸盐被还原,这表明缺少硝酸盐还原所需的一种电子载体。向生长培养基中添加血红素能使厌氧培养物还原硝酸盐。

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