Rowlinson Marie-Claire, Lebourgeois Phyllis, Ward Kevin, Song Yuli, Finegold Sydney M, Bruckner David A
Department of Pathology and Laboratory Medicine, UCLA Medical Center, Los Angeles, California 90095, USA.
J Clin Microbiol. 2006 Mar;44(3):857-60. doi: 10.1128/JCM.44.3.857-860.2006.
Staphylococcus epidermidis is a well-characterized, nonfastidious, aerobic gram-positive coccus commonly isolated in the clinical microbiology laboratory. Although coagulase-negative staphylococci, including Staphylococcus epidermidis, are often considered a contaminant in the clinical laboratory, an increasing number of reports describe their pathogenesis, in particular in infections of prosthetic devices. This article describes the isolation of a strictly anaerobic strain of Staphylococcus epidermidis in pure culture from the site of an infected prosthetic hip. This isolate was unique in that it grew only under strictly anaerobic conditions. Initially, the isolate was thought to be a known anaerobic gram-positive coccus. However, certain key biochemical and antimicrobial tests performed as part of the standard laboratory identification procedure were not consistent with results expected for any known anaerobic gram-positive coccus; the isolate was catalase positive and metronidazole and penicillin resistant. This isolate was characterized by further biochemical analysis, antimicrobial testing, and nucleic acid sequencing. This paper presents the first documented isolation of a strictly anaerobic Staphylococcus epidermidis strain, confirmed by rpoB gene sequencing.
表皮葡萄球菌是一种特征明确、非苛求性、需氧的革兰氏阳性球菌，在临床微生物实验室中经常分离得到。尽管包括表皮葡萄球菌在内的凝固酶阴性葡萄球菌在临床实验室中常被视为污染物，但越来越多的报告描述了它们的致病机制，尤其是在人工装置感染方面。本文描述了从感染的人工髋关节部位纯培养分离出一株严格厌氧的表皮葡萄球菌。该分离株的独特之处在于它仅在严格厌氧条件下生长。最初，该分离株被认为是一种已知的厌氧革兰氏阳性球菌。然而，作为标准实验室鉴定程序一部分进行的某些关键生化和抗菌测试结果与任何已知厌氧革兰氏阳性球菌预期的结果不一致；该分离株过氧化氢酶阳性，对甲硝唑和青霉素耐药。通过进一步的生化分析、抗菌测试和核酸测序对该分离株进行了鉴定。本文首次记录了经rpoB基因测序证实的严格厌氧表皮葡萄球菌菌株的分离。
