CHATTERJEE B R, WILLIAMS R P
J Bacteriol. 1963 Apr;85(4):838-41. doi: 10.1128/jb.85.4.838-841.1963.
Chatterjee, B. R. (Baylor University College of Medicine, Houston, Texas), and Robert P. Williams. Preparation of spheroplasts from Vibrio comma. J. Bacteriol. 85:838-841. 1963.-Spheroplasts were prepared from several strains of Vibrio comma by lysozyme treatment combined with freezing and thawing of the organisms. The optimal concentration of lysozyme was 50 mug/ml, although some spheroplasts formed at a concentration of 10 mug/ml. Higher concentrations (200 mug/ml) caused lysis of cells along with spheroplast formation. Treatment was carried out in broth cultures containing 15% sucrose, and if the osmotic tension was lowered the spheroplasts lysed. Some motile, spherical cells were present in every preparation. Addition of 3% glycine to broth cultures resulted in rapid transformation of the vibrios into large, spherical bodies. However, these were actively motile, and were not sensitive to a lower osmotic tension. Therefore, they could not be considered as spheroplasts.
查特吉，B. R.（得克萨斯州休斯顿贝勒医学院）和罗伯特·P. 威廉姆斯。从霍乱弧菌制备原生质球。《细菌学杂志》85:838 - 841。1963年。——通过溶菌酶处理并结合生物体的冻融，从几株霍乱弧菌制备了原生质球。溶菌酶的最佳浓度为50微克/毫升，不过在10微克/毫升的浓度下也形成了一些原生质球。更高的浓度（200微克/毫升）会导致细胞裂解并同时形成原生质球。处理在含有15%蔗糖的肉汤培养物中进行，如果渗透压降低，原生质球就会裂解。每次制备物中都存在一些有运动能力的球形细胞。向肉汤培养物中添加3%的甘氨酸会导致弧菌迅速转变为大型球形物体。然而，这些物体具有活跃的运动能力，并且对较低的渗透压不敏感。因此，它们不能被视为原生质球。
