Hsu J C, Chuang L Y, Chang C C
Department of Food Engineering, Tungfang Junior College of Technology and Commerce, Kaohsiung City, Taiwan, Republic of China.
Gaoxiong Yi Xue Ke Xue Za Zhi. 1992 Jun;8(6):320-6.
Bungarus multicinctus venom was incorporated into sphingomyelin-cholesterol liposomes which were stabilized with osmium tetroxide. The lethal toxicity of B. multicinctus venom in osmicated liposomes greatly decreased to 1.03% that of crude venom. Rabbits were divided into four groups for immunization. Group A was immunized with crude venom, group B and group C with osmicated liposomes, and group D with osmicated liposomes followed by the crude venom. Immunization was performed in the presence of Freund's adjuvant except for group B. The antibody levels during the course of immunization were measured by ELISA and the contents of precipitating antibody were determined by quantitative precipitin reactions, while that of non-precipitating antibody was determined by affinity chromatography. Although the immune response was not increased by incorporating lipopolysaccharide immunostimulant, the specific neutralizing capacity of purified antibody from the pooled antisera of group D was more potent than that of the others. The almost non-toxic osmicated liposomes of B. multicinctus venom may be useful immunogens for the production of antiserum for treatment of snake bites.
银环蛇毒被包入用四氧化锇稳定的鞘磷脂 - 胆固醇脂质体中。经四氧化锇处理的脂质体中银环蛇毒的致死毒性大幅降低至粗毒的1.03%。将兔子分为四组进行免疫。A组用粗毒免疫，B组和C组用经四氧化锇处理的脂质体免疫，D组先用经四氧化锇处理的脂质体免疫，随后再用粗毒免疫。除B组外，均在弗氏佐剂存在的情况下进行免疫。免疫过程中的抗体水平通过ELISA测定，沉淀抗体含量通过定量沉淀反应确定，而非沉淀抗体含量通过亲和层析测定。尽管加入脂多糖免疫刺激剂并未增强免疫反应，但D组混合抗血清中纯化抗体的特异性中和能力比其他组更强。几乎无毒的经四氧化锇处理的银环蛇毒脂质体可能是用于生产治疗蛇咬伤抗血清的有用免疫原。
