New R R, Theakston R D, Zumbuehl O, Iddon D, Friend J
Toxicon. 1985;23(2):215-9. doi: 10.1016/0041-0101(85)90144-8.
A method is described which produces a high, permanent antibody response following a single injection of venom. Animals (mice, rabbits, sheep) were given intravenous, subcutaneous or orally administered Nigerian Echis carinatus (carpet viper) venom which had been incorporated into sphingomyelin-cholesterol liposomes whose membranes had been stabilized by cross-linking adjacent molecules of sphingomyelin using osmium tetroxide. Before use the preparations were thoroughly dialysed to remove any unbound osmium tetroxide. Antibody levels were estimated using enzyme immunoassay. Venom treated in this way and administered i.v. or s.c. produced a powerful, sustained and protective antibody response lasting for the lifetime of a mouse. We also report the development of significant antibody responses after oral administration of liposome-entrapped but not free venom.
本文描述了一种方法，该方法在单次注射毒液后可产生高且持久的抗体反应。给动物（小鼠、兔子、绵羊）静脉内、皮下或口服给予已掺入鞘磷脂 - 胆固醇脂质体的尼日利亚锯鳞蝰蛇毒，该脂质体的膜通过使用四氧化锇交联相邻的鞘磷脂分子而得以稳定。在使用前，将制剂充分透析以去除任何未结合的四氧化锇。使用酶免疫测定法估计抗体水平。以这种方式处理并静脉内或皮下给药的毒液产生了强大、持续且具有保护性的抗体反应，持续小鼠的一生。我们还报告了口服脂质体包裹的毒液而非游离毒液后产生显著抗体反应的情况。
