Freitas T V, Tavares A P, Theakston R D, Laing G, New R R
Centro de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, Brasil.
Toxicon. 1989;27(3):341-7. doi: 10.1016/0041-0101(89)90181-5.
Crotalus durissus venom has been described as a weak antigen when injected in combination with Freund's complete adjuvant during the course of traditional methods of equine immunisation. Antibody production is slow and unpredictable, with a wide variation in individual responses. In this experimental study, C. durissus venom was incorporated into stabilised sphingomyelin-cholesterol liposomes both in the presence and absence of lipopolysaccharide immunostimulant and injected by both i.v. and s.c. routes into mice and rabbits. A rapid, sustained and protective immune response was obtained following a single injection of these preparations in mice. Antibody levels were estimated using enzyme-linked immunosorbent assay (ELISA), and the protective effect was evaluated by subsequent challenge with a subcutaneous minimum lethal dose of the venom. Results indicated that the immune response was significantly potentiated by the presence of immunostimulant in the venom liposomes. The use of C. durissus venom liposomes should be a useful tool for the immunisation of animals both in experimental and commercial procedures.
在传统的马免疫方法过程中，当与弗氏完全佐剂联合注射时，杜氏响尾蛇毒液被描述为一种弱抗原。抗体产生缓慢且不可预测，个体反应差异很大。在这项实验研究中，杜氏响尾蛇毒液在有和没有脂多糖免疫刺激剂的情况下被掺入稳定化的鞘磷脂 - 胆固醇脂质体中，并通过静脉内和皮下途径注射到小鼠和兔子体内。在小鼠单次注射这些制剂后获得了快速、持续和保护性的免疫反应。使用酶联免疫吸附测定（ELISA）估计抗体水平，并通过随后皮下注射最小致死剂量的毒液进行攻击来评估保护作用。结果表明，毒液脂质体中免疫刺激剂的存在显著增强了免疫反应。杜氏响尾蛇毒液脂质体的使用在实验和商业程序中都应该是动物免疫的有用工具。
