Isolation of dengue type 3 from mosquitoes in Rangoon.

A virus was isolated in suckling mice from Aedes aegypti collected from Rangoon area, during the month of July 1969. The virus was identified as a member of Group B arbovirus dengue type 3, by means of its ability to agglutinate goose erythrocyte at pH 6.6, haemagglutination inhibition and complement fixation tests, and its growth characteristics in Aedes albopictus cell cultures. The isolated dengue type 3 virus differs slightly from the Indian dengue type 3 virus in its ability to produce haemagglutinin of high titer from the early mouse passage, variation in the pH for optimum haemagglutination and ability to produce cytopathic effect in Vero cell cultures.