Fukunaga T, Okuno Y, Tadano M, Fukai K
Biken J. 1982 Sep;25(3):139-47.
One of the authors (Y.O.) of this paper was accidentally infected in the laboratory with dengue type 4 virus, isolated in Thailand in 1978 using the C6/36 clone of Singh's Aedes albopictus cells (SA). Two strains of the virus were isolated from Y.O.'s serum on the first day of illness using SA and suckling mouse brain (SMB). The SA-isolate and the infecting virus were neutralized with high efficiency by Y.O.'s convalescent sera, but the SMB-isolate was neutralized much less efficiently by the same sera. When the hosts of the isolates were exchanged (SA to SMB, and vice versa), the neutralization efficiencies were reversed. For analysis of this phenomenon, prototype dengue type 4 virus exclusively passed in SMB and the same type of virus passaged 10 times in SA were compared with the initial SA- and SMB-isolates in neutralization tests with sera from patients with dengue hemorrhagic fever (DHF) obtained in Thailand. The two strains of the prototype virus and the SA-isolate had similar high reactivities, but the SMB-isolate had low reactivity with sera of the DHF patients.
本文的一位作者（Y.O.）在实验室意外感染了1978年于泰国使用辛格白纹伊蚊细胞（SA）的C6/36克隆株分离出的4型登革病毒。在发病第一天，使用SA和乳鼠脑（SMB）从Y.O.的血清中分离出两株病毒。SA分离株和感染病毒被Y.O.的恢复期血清高效中和，但相同血清对SMB分离株的中和效率要低得多。当分离株的宿主互换（SA换为SMB，反之亦然）时，中和效率发生逆转。为分析此现象，在中和试验中，将仅在SMB中传代的4型登革病毒原型株和在SA中传代10次的同一型病毒与从泰国登革出血热（DHF）患者血清中获得的初始SA和SMB分离株进行比较。两株原型病毒和SA分离株具有相似的高反应性，但SMB分离株与DHF患者血清的反应性较低。
